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2024 PP Systems
Innovator’s Travel Award

Application Deadline: April 1, 2024

Know an outstanding postdoc or graduate student involved in exciting leaf gas exchange and/or soil & net canopy flux measurements?

If you or someone you know is involved in exciting leaf gas exchange and/or soil and net canopy flux research and would like to present a poster and/or presentation at an upcoming conference or workshop that includes the use of our equipment, PP Systems may be able to help with travel expenses!

Our goal with these awards is to support the next generation of scientists in sharing their research with the world.

PP Systems Innovator's Travel Award Criteria

  • Be an active PP Systems user
  • Have an accepted poster or talk at an upcoming conference in 2024 that includes the use of at least one of our instruments as part of the research to be presented
  • Be active on social media (via your own lab blog, Twitter, Instagram, etc.)
  • Submit a 2-minute video telling us a bit about yourself, your research, and why you believe you would benefit from this travel award in regard to your research
  • Receive a written recommendation from your professor or supervisor
  • Create a brief (one-minute) follow-up video about how they benefited from attending the conference

Each recipient receives:

  • $1,000 to be used for travel expenses
  • Opportunity to work with PP Systems on application notes and possible webinar related to your research
  • Access to our scientific and technical staff
  • Support and promotion of your research, poster, and presentations

2023 PP Systems Innovator’s Travel Award Recipients

We are committed to continue to refine the role of instrumentation as a seamless extension of the thought process of every student of science, providing the freedom to focus on the valuable work to be done.

Suyun Nam

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Suyun Nam

University of Georgia | Dept of Horticulture
Professor/supervisor: Marc van Iersel

Research

Conventional greenhouse lighting control systems typically adjust supplemental lighting intensity based on timers or sunlight levels. However, as temperature conditions significantly affect photosynthetic performance, both light intensity and temperature should be considered for optimal light use efficiency. In this study, we aimed to investigate the effects of temperature and light intensity, as well as their interaction on photosynthesis, and to evaluate the acclimation processes to these factors. Four-week-old lettuce plants grown at 25 ℃ were transferred to a growth chamber with one of three temperature conditions (15, 25, and 35 ℃) and grown for a week under a photosynthetic photon flux density (PPFD) of either 150 or 500 µmol·m-2·s-1. Chlorophyll fluorescence data were collected every 30 minutes during the day. Photosynthesis, chlorophyll content index, starch, and soluble sugar contents were measured on 2 and 7 days after the treatment (DAT). Under 150 µmol·m-2·s-1, the quantum yield of photosystem II (φPSII) decreased over time, whereas φPSII increased under 500 µmol·m-2·s-1 by acclimating to the light intensities. Compared to 25 ℃, the dark-adapted φPSII (Fv/Fm), an indicator of photoinhibition, decreased gradually at 35 ℃. In particular, the combination of 35 ℃ and 500 µmol·m-2·s-1 resulted in extreme stress on the plants, causing wilting and loss of chlorophyll, with plants failing to acclimate to this high PPFD/high-temperature combination. However, the Fv/Fm decreased significantly on 2 DAT at 15 ℃ under 500 µmol·m-2·s-1, but it later recovered due to acclimation, even though the plants had lower overall φPSII during the experiment. This study suggests that plants use light energy less efficiently under low temperature and high light conditions, but they can acclimate to these conditions over time. But continuous high-temperature stress can induce excessive photoinhibition. Thus, supplemental lighting needs to be precisely controlled based on both temperature conditions and the acclimation process. Chlorophyll fluorescence measurements may provide a tool to do so.
SCRI funded all or part of the research associated with this abstract
Suyun Nam

Application Video

Conference Experience

Aline de Camargo Santos

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Aline de Camargo Santos

University of Florida | Dept of Horticultural Sciences
Professor/supervisor: Bruce Schaffer

Research

Salinity is a major cause of abiotic stress to crops, including papaya (Carica papaya L.), one of the most cultivated fruit crops in tropical and subtropical regions. Two experiments were conducted to assess the response of ‘Red Lady’ papaya plants to salinity stress. In both experiments, salt stress was imposed by adding different concentrations of Instant Ocean® to the irrigation water. In the first experiment, 5-month-old plants were subjected to electrical conductivity (ECw) levels in the irrigation water of 0, 1, 2, 3, 4, or 6 dS m-1 by manually irrigating them to field capacity every other day. To assess plant stress, single-leaf gas exchange [net CO2 assimilation (leaf-A), transpiration (leaf-Tr), and stomatal conductance of water vapor (leaf-gs)] was measured weekly for plants in each treatment. Plants were harvested after eight weeks and leaf, stem, and root dry weights were determined for all plants. Six weeks after treatments began, there was a 40-50% reduction in leaf-A, leaf-Tr, and leaf-gs for plants subjected to 6 dS m-1, whereas there was no significant difference in leaf gas exchange among any of the other treatments. There was no significant effect of ECw on leaf, stem, or root dry weights. A second experiment was conducted using a high-throughput automated plant phenotyping system to apply treatments and assess salinity stress. In this experiment, 3-month-old plants were exposed to ECw levels of 0, 2, 4, or 8 dS m-1 applied daily via in-pot drippers. Whole-plant transpiration (plant-Tr), root water uptake, plant water use, and whole-plant biomass accumulation were measured continuously by the phenotyping system. There was a decrease in plant-Tr of 13, 25, and 55% for ECw levels of 2, 4, and 8 dS m-1, respectively, compared to the control treatment. The salt treatment also affected root water uptake, with decreases of 40, 70, and 74% for ECw levels of 2, 4, and 8 dS m-1, respectively, compared to the control treatment. Plant biomass was reduced by 16% at the ECw level of 8 dS m-1, while the other treatments had similar biomass to the control treatment. Both experiments indicated that ‘Red Lady’ papaya can tolerate salinity levels of up to 6-8 dS m-1. Assessing salinity stress of papaya using periodic single-leaf gas exchange measurements or continuous whole-plant physiological measurements were both effective for assessing papaya responses to salinity.

Aline de Camargo Santos

Application Video

Conference Experience

2019 PP Systems Innovator’s Travel Award Recipients

Jun Liu UGA Horticulture

Jun Liu

University of Georgia | Horticulture Department
Professor/supervisor: Marc van Iersel

Green Light Benefit Photosynthesis at High Light Level | ASHS 2019

Marc van Iersel Horticulture Physiology Lab

Research

Under our ultimate goal of improving profitability for controlled environment agriculture (CEA) by smarter lighting strategy, my research focuses on light quality of supplemental lighting.

There are many aspects of supplemental lighting that can be modified, for example, light intensity, photoperiod, and light quality. Optimized lighting strategy can help CEA growers reduce production costs, increase crop yield and quality, hasten or delay crop maturation time to meet market window, and overall increase profitability. Optimization of lighting strategy, however, requires an extensive understanding of spectral effects on photosynthesis and crop performance.

Part of my light quality research focuses on the benefit of green light on carbon fixation. Traditionally, green light is considered to be less photosynthetically active than red and blue light. However, green light can penetrate deeper into leaves and engage more cells for photosynthesis. Therefore it can be used more efficiently especially at high PPFD. To characterize the interactive effect of wavelength and intensity of growth lights on photosynthesis, I compared effects on photosynthesis of red, green and blue lights as well as red/green and blue/green mixtures at different ratios on ‘Green Tower’ lettuce (Lactuca sativa). At PPFD of 200 µmol m-2 s-1, red light and 80% red+20% green resulted in the highest photosynthetic rates, while blue, green and mixtures of these two yielded the lowest photosynthetic rates. At high PPFD (1000 mol m-2 s-1), red and green lights have comparably high photosynthetic rates, when blue light and its mixtures still have relatively low photosynthetic rates. Also, green light was efficient at up-regulating Rubisco activity.

In conclusion, green light is more effective at driving photosynthesis at high PPFD. This knowledge could lead to refined design of LED growing lights that will enhance the light use efficiency, cut back on energy consumption of CEA, help the industry increase profitability.

Application Video

Conference Experience

Raiza Castill0 UFL TREC

Raiza Castillo

University of Florida | Tropical Research and Educational Center
Professor/supervisor: Bruce Schaffer

Use of Stable Carbon Isotopes to Assess Photosynthetic Response of Redbay Trees to Laurel Wilt | ASHS 2019

Schaffer Tropical Plant Ecophysiology Lab

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Research

My research involves assessing the effects of Laurel wilt disease as it relates to the physiology of avocado cultivars and other related tree species. Laurel wilt is caused by Raffaelea lauricola, an aggressive fungal pathogen transmitted by bark beetles. Since its first report in 2002, laurel wilt has caused the death of millions of redbay and has become a major concern to Florida’s avocado industry. Florida’s avocado production is based in avocados from the West Indian race or West Indian hybrids, which have been proven to be far less tolerant to laurel wilt when compared with the Mexican and Guatemalan race. Infected trees with laurel wilt display a variety of internal and external disease symptoms including wilted and necrotic canopy and sapwood discoloration. After infection, some plants exhibited increased cell permeability, alterations in water use and photosynthesis as well as histological changes like the formation of gels, gums, and tyloses that occluded the xylem. For this reason, the overall goal of my research is to understand the relative physiological and anatomical differences among different avocado cultivars and forest trees to identify cultivars that are the most tolerant to laurel wilt infection as well as to understand the basis for this tolerance. Physiological measurements include periodic determinations of net CO2 assimilation (photosynthesis), transpiration, stomatal conductance, chlorophyll fluorescence, leaf chlorophyll concentration, xylem sap flow, hydraulic conductance and stem anatomy among different seedling and clonal avocado. My last experiment involved the use of carbon isotopes to determine if the decrease in photosynthesis of plants with laurel wilt is caused by restricting CO2 entry into the plant through the stomata or if there are other physiological factors involved. I will be presenting my findings at ASHS 2019.

Rebekah Davis | Mason Lab

Rebekah Davis

University of Central Florida | Biology Department
Professor/supervisor: Chase Mason

Photosynthetic Variation Among Cultivated Sunflower | Botany 2019

Mason Lab

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Research

Cultivated sunflower is the fourth most important oilseed crop globally and exhibits a wide phenotypic diversity across the germplasm. Using a core set of twelve inbred lines that capture approximately 50% of the allelic diversity across cultivated sunflower germplasm collections, the CIRAS-3 portable photosynthesis system was used to measure rapid A/Ci curves, generating key metrics of photosynthetic physiology like maximum rate of carboxylation, maximum rate of electron transport, maximum assimilation rate, and CO2 compensation point. These measured photosynthetic traits will be used to ground-truth potential high-throughput proxies for these traits, including hyperspectral reflectance and chlorophyll fluorescence. We are currently assessing the utility of hyperspectral reflectance for predicting A/Ci curve-derived photosynthetic traits across a wider panel of sunflower germplasm. Given the contribution of photosynthetic performance to overall yield and growth, these proxies, if validated, would make for fast and easy tools for phenotyping photosynthesis with applications to molecular breeding. Finally, a high-density genetic map is currently being used to perform genome-wide association mapping to describe the genetic architecture of these descriptors of photosynthetic physiology in cultivated sunflower. I will be presenting my research at Botany 2019.

Ji_Johng_Chen Youping Sun Lab

Ji-Jhong Chen

Utah State University | Plants, Soils and Climate Department
Professor/supervisor: Youping Sun

Comparing the Salt Tolerance of Three Landscape Plants Using Near Continuous Gradient Dosing System | ASHS 2019

Utah State University Center for Water-Efficient Landscaping

Read Ji-Jhong Chen’s Application Note: Evaluating Physiological Responses of Viburnum Species to Salinity Stress 

Research

My thesis research focuses on promoting the use of native plants for a water-efficient landscape. Developing sustainable horticultural practices for growing native plants in low-water-use landscapes will assist in water conservation and enhance environmental stewardship. I will use symbiotic bacteria to improve the adaptability and performance of two native plants [Ceanothus velutinus (snowbrush ceanothus) and Shepherdia rotundifolia (roundleaf buffaloberry)] in an urban landscape. These plants are actinorhizal plants and do not perform well in an urban landscape, which might be caused by the ineffective development of actinorhizal symbiosis with nitrogen-fixing actinobacteria (frankia). Frankia strains will be isolated from the soil and root samples of roundleaf buffaloberry and snowbrush ceanothus. The isolated frankia strains will inoculate roundleaf buffaloberry and snowbrush ceanothus plants that are propagated via cuttings and seeds with the hope to enhance their performance. Plant photosynthesis of inoculated plants in greenhouse or field environment will be investigated.

Additionally, I have investigated the salt tolerance of three common landscape plants [Hibiscus syriacus (rose of sharon), Physocarpus opulifolius (ninebark), and Spiraea japonica (japanese spirea)] using a near-continuous gradient dosing (NCGD) system. The innovative NCGD system allows researchers to evaluate a large number of plants for salt tolerance with multiple treatments, more flexibility, and reduced efforts of irrigation. The NCGD system delivered saline solutions at electrical conductivity (EC) levels ranging from 0.8 dS·m-1 to 6.4 dS·m-1. The salt stress negatively impacted plant growth and photosynthesis as EC levels increased. The salinity threshold defined as 50% loss of shoot dry weight was 5.4 and 4.6 dS·m-1, respectively, for ninebark and japanese spirea, however not determined for rose of Sharon which is the most salt tolerant species in the study. I am looking forward to presenting the detailed results of my research at the American Society for Horticultural Science 2019 annual conference in July 2019.

Katuwal UGA CIRAS-3

Krishna Katuwal

University of Georgia | Crop and Soil Sciences Department
Professor/supervisor: David Jespersen

Drought tolerance in seashore paspalum ecotypes collected from different climatic zones | ASA, CSSA, & SSSA 2019

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Research

Turfgrasses are a vital part of human lives since they provide several services including recreational, aesthetic and environmental services. The turfgrass industry is one of the fastest growing segments of U.S. agriculture with an annual economic value of $35 billion (Huang et al., 2014). However, drought is one of the major constraints to turfgrass production that causes severe damage each year (Dai, 2013). There is a need to improve understanding of turfgrass drought tolerance mechanisms for its sustainable production. The objective of our study was to enhance understanding of turfgrass drought tolerance mechanisms and identify drought tolerant turfgrass cultivars using physiological and molecular approaches. Fifteen genotypes of seashore paspalum were grown under two different water treatments: well-watered and water-stressed for 20 days. They were assessed using morpho-physiological parameters including gas exchange parameters measured using CIRAS 3 to identify drought tolerant and sensitive genotypes. Identified drought tolerant genotypes had greater photosynthetic rates, osmotic adjustments and leaf water use efficiencies compared to sensitive genotypes after drought stress treatment application. However, transpiration rates and stomatal conductance were lower in tolerant genotypes compared to sensitive ones indicating better ability of tolerant genotypes to conserve water by stomatal regulation under drought stress. Identified drought tolerant and sensitive genotypes will be further grown under fully watered and water stressed condition for proteomic and metabolomic analysis in the future. Leaf and root samples will be used to extract proteins and metabolites and extracted proteins and metabolites will be identified using mass spectrometry coupled with protein and metabolite databases. Identified proteins and metabolites will be compared to determine their differential expression pattern among seashore paspalum genotypes. Greatly expressed proteins and metabolites might have involved in improving drought tolerance mechanisms of drought-tolerant seashore paspalum genotypes.

I will be presenting my research at the 2019 annual Crop Science Society of America meeting.