Hansatech Instruments light sources have been designed to accompany the range of oxygen electrode chambers and control units in order to allow optimum performance and repeatability of measurements of photosynthesis and respiration. Light sources are positioned either directly or connected via fibre-optic cables to optical ports on the electrode chambers ensuring optimum sample illumination.
Hansatech Instruments LED light sources provide red illumination centered on 650nm with an HBW (Half Band Width) of ± 25nm which is automatically controlled via the Oxylab control unit. LED’s have the advantage of low heat output, rugged construction, long life, intensity adjustment without the need for optical filters and a fast rise to full intensity (milliseconds) negating the need for electronic shutters in fast fluorescence work.
The LS2 high intensity white light source contains a cooling fan, infra-red reducing “Hot mirror” and optics to provide light with minimum variation of intensity across the beam and little divergence from parallel. Automatic intensity control is not possible with the LS2. Attenuation of the light intensity is achieved by placing combinations of neutral density filters across the beam.
Oxytherm+ is supplied in 2 versions; Oxytherm+R (Respiration) and Oxytherm+P (Photosynthesis). Each version is fitted with an advanced electronic oxygen electrode chamber with a specific feature set to suit the intended purpose.
Both versions offer precision sample and sensor temperature control between 3 – 40°C via an integral Peltier element. Chamber temperature is configured from within the OxyTrace+ Windows software with actual chamber temperature indicated on an LCD display mounted on the front panel of the Oxytherm+ electrode chamber. A tricolour LED indicates if the chamber is cooling (blue), heating (red) or at set temperature (green).
Liquid Phase Photosynthesis Systems
The Oxytherm+P has additional functionality to provide optimum suitability for photosynthesis studies. 2 high-intensity white LED light sources are mounted against the outer wall of the reaction vessel. This provides uniform illumination of samples between 1 and 2ml in volume up to 4,000μmols m-2 s-1. (blue), heating (red) or at set temperature (green).
Chlorolab 2 provides a sophisticated system for the advanced study of respiration and photosynthesis from liquid samples under automated illumination from red (660nm) LED light. The system provides the ability to automate the acquisition of oxygen evolution/uptake rate over a user-defined light intensity rate and to determine the apparent quantum yield.
Chlorolab 3 provides a sophisticated system for the advanced study of respiration and photosynthesis from larger volume liquid samples (to 20ml) under automated illumination from red (660nm) LED light. The system provides the ability to automate the acquisition of oxygen evolution/uptake rate over a user-defined light intensity rate and to determine the apparent quantum yield. Chlorolab 3 is particularly suitable for macroalgal studies in sea water including P/I curves by automated determination of oxygen evolution and uptake rates at a range of user-defined light intensities to determine apparent quantum yield.
Gas Phase Photosynthesis
Leaflab 2 offers the capability for advanced studies of photosynthesis and respiration from leaf-discs. The system provides computerized control of the oxygen signal with automated control of a red (660nm) LED light source array. 32 bit Windows® software functions provide automated measurement of the rate of oxygen evolution/uptake at a range of user-defined light intensities in order to determine apparent quantum yield.