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Chlorophyll Fluorescence Overview

The capacity of a plant to carry out photochemistry is limited and will depend upon a range of factors including stresses caused by environmental conditions. Absorbed light energy in excess of that used for photochemistry must be effectively dissipated by non-photochemical processes. Such processes include the emission of heat and re-emission of small but diagnostically significant amounts of the absorbed radiation as longer wavelength red/far-red light energy. This re-emission of light is termed chlorophyll fluorescence. Although chlorophyll fluorescence emission from whole leaf systems is too weak to be viewed with the naked eye, it can be observed from illuminated extracts of a chlorophyll solution. Peak chlorophyll fluorescence occurs in the red region of the spectrum (685 nm) and extends into the infra-red region to around 800 nm.

Each of these processes operate in direct competition for a finite pool of absorbed energy, any change in energy utilization by one process produces a complementary change in the others. This fact enables chlorophyll fluorescence to be used as a rapid and reliable non-invasive probe of photochemistry. Specialist equipment is required for the analysis of the chlorophyll fluorescence signature. A Chlorophyll Fluorometer is designed specifically to detect the chlorophyll fluorescence emission from a sample. There are several different types of chlorophyll fluorometers available.

Hansatech Instruments design and manufacture chlorophyll fluorometers based around 2 different measurement techniques, Pulse Modulated Chlorophyll Fluorometers and Continuous Excitation or Prompt Chlorophyll Fluorometers. These chlorophyll fluorometers cover a wide range of applications in photosynthesis research programs. To learn more about these techniques and to determine which instrument best suits your application, please get in contact with PP Systems to speak to a products specialist. We look forward to being of assistance.

Pulse Modulated Fluorometers

FMS2 Field Fluorescence Monitoring System

FMS2 Field Fluorescence Monitoring System from Hansatech Instruments

The FMS2 is a field portable pulse modulated chlorophyll fluorometer. The system is highly portable and fully self-contained.  A system of field interchangeable batteries provides power for a full day of field work. A multi-charger system allows full recharge of the batteries overnight.  For information on the use of the FMS2 with the CIRAS for simultaneous leaf gas exchange and chlorophyll fluorescence measurements, contact PP Systems.

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Product Features

  • Pulse modulated system
  • Compact field design
  • Temperature compensated electronics
  • Programmable by Hansatech Scripting Language (HSL)
  • Leaf-clip with integral PAR/temperature sensor
  • Field swappable battery system
  • Windows® data acquisition & data analysis software

FMS1 Fluorescence Monitoring System

FMS1 Fluorescence Monitoring System for the lab from Hansatech Instruments

The FMS1 is a pulse modulated chlorophyll fluorometer operating from a mains power supply for laboratory use. The system may also be operated from an external 12V D.C. power supply or high capacity battery for near-field conditions. For information on the use of the FMS1 with the CIRAS for simultaneous leaf gas exchange and chlorophyll fluorescence measurements, contact PP Systems.

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Product Features

  • Pulse-modulated system
  • Integral LED & tungsten-halogen light sources
  • External device control interface
  • Programmable by Hansatech Scripting Language (HSL)
  • Optional leaf-clip with integral PAR/temperature sensor
  • Fiber optic for incorporation into O2 electrode units & IRGA
  • Windows® data acquisition & data analysis software

Continuous Excitation Systems

Pocket PEA

Pocket PEA Fluorometer from Hansatech Instruments

The Pocket PEA Chlorophyll Fluorimeter is suitable for teaching, research & a wide variety of commercial applications. The robust yet compact hand-held design provides ease of use & reliable operation.

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Product Features

  • Ultra-portable chlorophyll fluorometer
  • Rapid screening capability with single button operation & storage of up to 200 full data sets
  • Automatic calculation of parameters including Fv/Fm & OJIP analysis
  • Robust enclosure with sealed, high intensity optics
  • 100kHz sampling frequency with 16bit resolution
  • Bluetooth wireless data transfer as standard
  • Powerful Windows® data transfer & analysis software included

Handy PEA

Handy-PEA Fluorometer from Hansatech Instruments

Handy-PEA is a compact, highly portable continuous-excitation type chlorophyll fluorescence analyzer. The rugged yet lightweight design makes Handy-PEA particularly suitable for rapid, large scale screening of samples in the field or greenhouse in both research and teaching applications.

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Product Features

  • Compact (170 x 85 x 40mm), lightweight (565gms)
  • Large-scale screening capacity up to 1000 full trace data files
  • High time resolution detection for discrimination of fast chlorophyll fluorescence induction kinetics
  • Saturating high intensity focused LED array for accurate determination of Fmax
  • Upload user-defined, repeatable protocols for automatic field execution
  • Interchangeable sensor unit cables with lengths of up to 20 metres
  • Powerful Windows® data transfer & analysis software included

M-PEA

MPEA Chlorophyll Fluorescence System from Hansatech Instruments

The M-PEA (Multi-Function Plant Efficiency Analyzer) combines high quality fast fluorescence kinetic and P700+ absorbance studies with ground-breaking Delayed Fluorescence (DF) measurements providing one of the most comprehensive systems for the investigation of plant photosynthetic efficiency available.

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Fluorescence kinetics from M-PEA

Screenshot demonstrating prompt fluorescence and P700+ absorbance

Fluorescence kinetics from M-PEA

Screenshot demonstrating delayed fluorescence

Product Features

  • Advanced lab-based system for investigation of plant photosynthetic efficiency.
  • M-PEA-1 variant for prompt fluorescence and P700+ modulated absorbance measurements.
  • M-PEA-2 variant as M-PEA-1 with additional measurements of delayed fluorescence and leaf absorbtivity.
  • Sophisticated sensor unit with all optical emitters and detectors in robust, enclosed housing.
  • USB connection to a Windows® PC.
  • Comprehensive Windows® experimental design, data transfer and analysis software.